Authors Affiliation(s)
- 1Laboratoire PROTEE, EA – Université de Toulon, Toulon-La Garde 83957, FRANCE
- 2Inserm U927, Ischémie-reperfusion en transplantation rénale, Poitiers, FRANCE
- 3Laboratoire Velay, Puy en Velay, FRANCE
- 4UF Biosanté, Faculté des Sciences Fondamentales et Appliquées, 40 rue du recteur Pineau, Poitiers Cedex 86022, Université de Poitiers, FRANCE
Can J Biotech, Volume 2, Issue 3, Pages 116-123, DOI: https://doi.org/10.24870/cjb.2018-000123
Received: Jul 18, 2018; Revised: Oct 1, 2018; Accepted: Oct 10, 2018
Abstract
Background: Herbal plant extracts are a more common alternative to conventional medicine to treat sleep disorders and intermittent hypoxia. Notably, obstructive sleep apnea causes injuries similar to those observed in models of ischemia-reperfusion including the decrease of nitric oxide (NO) availability. Kidney transplantation in end-stage renal disease reverses the sleep apnea. The underlying mechanism linking hypoxia, sleep apnea, and renal protection remains to be defined at the cellular level.
Objective: The aim of this study was to demonstrate the safety and efficacy of herbal plant infusions with a potential for donating NO, to attenuation of damage induced during a hypoxia/reperfusion sequence, on kidney epithelial cells LLC-PK1.
Materials and Methods: Cell death (Lactate Dehydrogenase release assay) and a viability test (MTS assay) after 24 h of incubation with different concentrations of plant infusion were assessed using the LLC-PK1 cell line. Then, measurement of the breakdown product of NO (the NaNO2) and LDH assay were carried out after 24 h of hypoxia, followed by 4 h or 24 h of reperfusion.
Results: The effect of different dilutions of herbal plant infusion on the LLC-PK1 cell viability, after 24 h of incubation, was maximal at a 30% dilution compared to control. After 24 h of hypoxia, there was an increase of NaNO2 and thus of NO, and a concentration-dependent decrease of cell death. Similar results were observed after hypoxia followed by 4 h of reperfusion. These effects were always maximal at 50% dilution of plants infusion.
Conclusion: Safe infusion of plant extracts causes a dose-dependent increase of NO and has a protective effect against the cellular stress caused by hypoxia and reoxygenation. Since it has been demonstrated that there is a NO-dependent mechanism allowing the reduction of injuries induced by ischemia/reoxygenation process, such a mechanism could be responsible for our observations.
References
- Williamson, E.M. (2001) Synergy and other interactions in phytomedicines. Phytomedicine 8: 401-409. Crossref
- Saeed, S.A., Bloch, R.M. and Antonacci, D.J. (2007) Herbal and dietary supplements for treatment of anxiety disorders. Am Fam Physician 76: 549-556.
- Kinrys, G., Coleman, E. and Rothstein, E. (2009) Natural remedies for anxiety disorders: potential use and clinical applications. Depress Anxiety 26: 259-265. Crossref
- Sarris, J., Kavanagh, D.J. and Byrne, G. (2010) Adjuvant use of nutritional and herbal medicines with antidepressants, mood stabilizers and benzodiazepines. J Psychiatr Res 44: 32-41. Crossref
- Sharma A.,Cardoso-Taketa, A., Garcia, G.and Villarreal,M.L. (2012) A systematic updated review of scientifically tested selected plants used for anxiety disorders. Botanics: Targets and Therapy 2012: 21-39. Crossref
- Atkeson, A. and Jelic, S. (2008) Mechanisms of endothelial dysfunction in obstructive sleep apnea. Vasc Health Risk Manag 4: 1327-1335. Crossref
- Guix, F.X., Uribesalgo, I., Coma, M. and Munoz, F.J. (2005) The physiology and pathophysiology of nitric oxide in the brain. Prog Neurobiol 76: 126-152. Crossref
- Prabhakar, N.R. (2002) Sleep apneas:an oxidative stress? Am J Respir Crit Care Med 165: 859-860. Crossref
- Yang, X.H., Zhang, B.L., Gu, Y.H., Zhan, X.L., Guo, L.L. and Jin, H.M. (2018) Association of sleep disorders, chronic pain, and fatigue with survival in patients with chronic kidney disease: a meta-analysis of clinical trials. Sleep Med 51: 59-65. Crossref
- Perl, J., Unruh, M.L. and Chan, C.T. (2006) Sleep disorders in end-stage renal disease: ‘Markers of inadequate dialysis’? Kidney Int 70: 1687-1693. Crossref
- Johnston, J.G. and Pollock, D.M. (2018) Circadian regulation of renal function. Free Radic Biol Med 119: 93-107. Crossref
- Rivier, C.(2001) Role of gaseous neurotransmitters in the hypothalamic-pituitary-adrenal axis. Ann N Y Acad Sci 933:254-264. Crossref
- Datta, S., Patterson, E.H. and Siwek, D.F. (1997) Endogenous and exogenous nitric oxide in the pedunculopontine tegmentum induces sleep. Synapse 27: 69-78. Crossref
- Hars, B. (1999) Endogenous nitric oxide in the rat pons promotes sleep. Brain Res 816: 209-219. Crossref
- Cavas, M. and Navarro, J.F. (2006) Effects of selective neuronal nitric oxide synthase inhibition on sleep and wakefulness in the rat. Prog Neuro-Psychopharmacol Biol Psychiatry 30: 56-67. Crossref
- Lurie, A. (2011) Endothelial dysfunction in adults with obstructive sleep apnea. Adv Cardiol 46: 139-170. Crossref
- François, C., Fares, M., Baiocchi, C. and Maixent, J.M. (2015) Safety of Desmodium adscendens extract on hepatocyte and renal cells. Protective effect against oxidative stress. J Intercult Ethnopharmacol 4: 1-5. Crossref
- Bartels-Stringer, M., Verpalen, J.T., Wetzels, J.F., Russel, F.G. and Kramers, C. (2007) Iron chelation or anti-oxidants prevent renal cell damage in the rewarming phase after normoxic, but not hypoxic cold incubation. Cryobiology 54: 258-264. Crossref
- Fares, M., Armand, M., François, C. and Maixent, J.M. (2012) ω6/ω3 polyunsaturated fatty acid supplementations in renal cell model lead to a particular regulation through lipidome for preserved ω6/ω3 ratios. Cell Mol Biol (Noisy-le-grand) 58: OL1715-OL1719. Crossref
- Salahudeen, A.K., Huang, H., Joshi, M., Moore, N.A. and Jenkins, J.K. (2003) Involvement of themitochondrialpathway in cold storage and rewarming-associated apoptosis of human renal proximal tubular cells. Am J Transplant 3: 273-280. Crossref
- Koyama, I., Bulkley, G.B., Williams, G.M. and Im, M.J. (1985) The role of oxygen free radicals in mediating the reperfusion injury of cold-preserved ischemic kidneys. Transplantation 40: 590-595.
- Paller, M.S. (1992) Free radical-mediated postischemic injury in renal transplantation. Ren Fail 14: 257-260. Crossref
- Sakon, M., Ariyoshi, H., Umeshita, K. and Monden, M. (2002) Ischemia-reperfusion injury of the liver with special reference to calcium-dependent mechanisms. Surg Today 32: 1-12. Crossref
- Quintana, A., Rodriguez, J.V., Scandizzi, A. and Guibert, E.E.(2001) Effect of S-nitrosoglutathione (GSNO) added to the University of Wisconsin solution (UW): I) Morphological alteration during cold preservation/reperfusion of rat liver. Int J Surg Investig 2: 401-411.
- Quintana, A.B., Rodriguez, J.V., Scandizzi, A.L. and Guibert, E.E. (2003) The benefit of adding sodium nitroprusside (NPNa) or S-nitrosoglutathion (GSNO) to the University of Wisconsin solution (UW) to prevent morphological alterations during cold preservation/reperfusion of rat livers. Ann Hepatol 2: 84- 91.
- Nakano, A., Liu, G.S., Heusch, G., Downey, J.M. and Cohen, M.V. (2000) Exogenous nitric oxide can trigger a preconditioned state through a free radical mechanism, but endogenous nitric oxide is not a trigger of classical ischemic preconditioning. J Mol Cell Cardiol 32: 1159- 1167. Crossref
- Valdivielso, J.M., Crespo, C., Alonso, J.R., Martinez- Salgado, C., Eleno, N., Arevalo, M., Perez-Barriocanal, F. and Lopez-Novoa, J.M. (2001) Renal ischemia in the rat stimulates glomerular nitric oxide synthesis. Am J Physiol Regul Integr Comp Physiol 280: R771-R779. Crossref
- Liang, M. and Knox, F.G. (2000) Production and functional roles of nitric oxide in the proximal tubule. Am J Physiol Regul Integr Comp Physiol 278: R1117-R1124. Crossref
- Yu, L., Gengaro, P.E., Niederberger, M., Burke, T.J. and Schrier, R.W. (1994) Nitric oxide: a mediator in rat tubular hypoxia/reoxygenation injury. Proc Natl Acad Sci U S A 91: 1691-1695. Crossref
- Noiri, E., Peresleni, T., Miller, F. and Goligorsky, M.S. (1996) In vivo targeting of inducible NO synthase with oligodeoxynucleotides protects rat kidney against ischemia. J Clin Invest 97:2377-2383. Crossref
- Chiao,H., Kohda, Y., McLeroy, P., Craig, L., Housini, I. and Star, R.A. (1997) Alpha-melanocyte-stimulating hormone protects against renal injury after ischemia in mice and rats. J Clin Invest 99: 1165-1172. Crossref
- Garcia-Criado, F.J., Eleno, N., Santos-Benito, F., Valdunciel, J.J., Reverte, M., Lozano-Sanchez, F.S., Ludena, M.D., Gomez-Alonso, A. andLopez-Novoa, J.M. (1998) Protective effect of exogenous nitric oxide on the renal function and inflammatory response in a model of ischemia-reperfusion. Transplantation 66: 982-990. Crossref
- Katori, M., Tamaki, T., Takahashi, T., Tanaka, M., Kawamura, A. and Kakita, A. (2000) Prior induction of heat shock proteins by a nitric oxide donor attenuates cardiac ischemia/reperfusion injury in the rat. Transplantation 69: 2530-2537.
- Beckman, J.S. and Crow, J.P. (1993) Pathological implications of nitric oxide, superoxide and peroxynitrite formation. Biochem Soc Trans 21: 330-334. Crossref
- Southan, G.J. and Szabo, C. (1996) Selective pharmacological inhibition of distinct nitric oxide synthase isoforms. Biochem Pharmacol 51: 383-394. Crossref
- Pierre, S.V., Lesnik, P., Moreau, M., Bonello, L., Droy- Lefaix, M.T., Sennoune, S., Duran, M.J., Pressley, T.A., Sampol, J., Chapman, J. and Maixent, J.M. (2008) The standardized Ginkgo Biloba extract EGB-761 protects vascular endothelium exposed to oxidized low density lipoproteins. Cell Mol Biol 54: OL1032-OL1042.
- Braud, L., Peyre, L., deSousa, G., Armand, M., Rahmani, R. and Maixent, J.M. (2015) Effect of brewing duration on the antioxidant and hepatoprotective abilities of tea phenolic and alkaloid compounds in a t-BHP oxidative stress-induced rat hepatocyte model. Molecules 20: 14985-15002. Crossref
- Braud, L., Battault, S., Nascimento, A., Gaillard, S., de Sousa, G., Rahmani, R., Riva, C., Armand, M.,Maixent,J.M. and Reboul, C. (2017) Antioxidant properties of tea blunt ROS-dependent lipogenesis: beneficial effect on hepatic steatosis in a high fat-high sucrose diet NAFDL obese rat model. J Nutr Biochem 40: 95-104. Crossref