Bacterial Xylanase in Pseudomonas boreopolis LUQ1 is Highly Induced by Xylose

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  1. Chaoyang Lin1,2
  2. Zhicheng Shen1,
  3. Tingheng Zhu3,
  4. Wensheng Qin2*

Authors Affiliation(s)

  • 1Institute of Insect Science, Department of Plant Protection, Zhejiang University, Hangzhou 310058, CHINA
  • 2Department of Biology, Lakehead University, Thunder Bay, P7B5E1, Ontario, CANADA
  • 3College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, CHINA

Can J Biotech, Volume 1, Issue 3, Pages 73-79, DOI: https://doi.org/10.24870/cjb.2017-000112

Received: Sep 10, 2017; Revised: Oct 25, 2017; Accepted: Oct 26, 2017

Abstract

A xylanase producing bacterium was isolated from paper mill sludge in Thunder Bay, Canada. The newly isolated bacterium was identified as Pseudomonas boreopolis according to its 16S rRNA gene sequence and designated as LUQ1. The zymogram analysis indicated that there was one band of protein with xylanase activity, and the molecular weight of the enzyme was about 20 kDa. This xylanase works best at pH 6.0 and 65°C. The xylanase can be induced to express by xylose. The expression was enhanced on increasing the concentration of xylose, which reached its highest activity at 12 mM of xylose in fermentation. Wheat bran was the best carbon source in submerged fermentation. The highest xylanase activity of 25.61 U/ml was obtained at 96 h using wheat bran feedstock. When barley straw and wheat bran were used as feedstocks, the addition of 10 mM of xylose increased xylanase activity by ~50% and ~15%, respectively. The results showed that the strain LUQ1 has a great potential to produce xylanase for industrial applications.

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